7-Hydroxycoumarinyl arachidonate

7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2) . 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL) . MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573) . Release of 7-HC can be measured using a fluorometer[1][2][3].

Product Specifications

CAS Number

[161180-11-6]

Product Name Alternative

7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

MAGL; Phospholipase

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Applications

Metabolism-sugar/lipid metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/7-hydroxycoumarinyl-arachidonate.html

Concentration

55.73 mM * 400 μL in Ethanol

Purity

99.19

Solubility

10 mM in DMSO

Smiles

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(OC1=CC=C2C=CC(OC2=C1)=O)=O

Molecular Formula

C29H36O4

Molecular Weight

448.59

Precautions

H302, H315, H319, H335

References & Citations

[1]Yuren Wang, et al. A fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening. Assay Drug Dev Technol. 2008 Jun;6 (3) :387-93.|[2]Z Huang, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222 (1) :110-5.|[3]R T Pickard, et al. Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine. J Biol Chem. 1996 Aug 9;271 (32) :19225-31.