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CEBPZ Antibody

C/EBPZ (CCAAT/enhancer-binding protein Z), also known as CBF, CBF2, NOC1 or HSP-CBF is a 1,054 amino acid nuclear protein belonging to the CBF/MAK21 family. C/EBPZ stimulates transcription from the HSP70 and HSP40 promoters in a NF-Y dependent manner, requiring an intact NF-Y trimer which binds to the DNA. C/EBPZ is thought to be a potential tumor suppressor gene, and aberrant methylation of the C/EBPZ promoter may be involved with acute myeloid leukemia. The C/EBPZ gene is conserved in a wide number of species including chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish and C. elegans and is located on human chromosome 2. Chromosome 2, the second largest human chromosome, consists of 237 million bases encoding over 1,400 genes, comprising approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2.

Product Specifications

Specifications

Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q03701

Host

Mouse

Reactivity

Human

Immunogen

The protein domain of CEBPZ protein was used as the immunogen for the CEBPZ antibody.

Clonality

Monoclonal

Isotype

IgG2c

Clone

PCRP-CEBPZ-2D8

Applications

FACS, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CEBPZ antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CEBPZ antibody is available for research use only.

Storage Conditions

Aliquot the CEBPZ antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/cebpz-antibody-pcrp-cebpz-2d8-v4893

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CEBPZ antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Nucleus

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using CEBPZ Mouse Monoclonal (PCRP-CEBPZ-2D8) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
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