OIP-4 Antibody / PRAME

Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P78395

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 100-509) from the human protein was used as the immunogen for the OIP-4 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

PRAME/6928R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the OIP-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This OIP-4 antibody is available for research use only.

Storage Conditions

Aliquot the OIP-4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the OIP-4 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm, Cell membrane

Image Legend

IHC staining of FFPE human melanoma tissue with OIP-4 antibody (clone PRAME/6928R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.