Interferon regulatory factor 3 Antibody / IRF3

Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-Alpha and beta) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-Alpha and interferon-beta genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3gammap48 and IFN consensus sequence-binding protein (ICSBP) .

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q14653

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human IRF3 protein was used as the immunogen for the Interferon regulatory factor 3 antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

PCRP-IRF3-4D7

Applications

FACS, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Interferon regulatory factor 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Interferon regulatory factor 3 antibody is available for research use only.

Storage Conditions

Aliquot the Interferon regulatory factor 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Interferon regulatory factor 3 antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

Flow cytometry testing of PFA-fixed human HeLa cells with Interferon regulatory factor 3 antibody (clone PCRP-IRF3-4D7) followed by goat anti-mouse IgG-CF488 (blue) ; Red = unstained cells.