Von Willebrand Factor Antibody

Von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P04275

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human VWF protein was used as the immunogen for the von Willebrand Factor antibody.

Clonality

Monoclonal

Isotype

IgG1

Clone

VWF/4458

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the von Willebrand Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This von Willebrand Factor antibody is available for research use only.

Storage Conditions

Aliquot the von Willebrand Factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.

Location

Secreted, Cytoplasm

Image Legend

IHC staining of FFPE human tonsil tissue with vWF antibody (clone VWF/4458) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.