KChIP2 Antibody / KCNIP2

In the brain and heart, rapidly inactivating (A-type) voltage-gated potassium (Kv) currents control the excitability of neurons and cardiac myocytes. KChIPs are Kv channel-interacting proteins that bind to the cytoplasmic amino termini of Kv4Alpha-subunits and are integral components of native Kv4 channel complexes. KChIP family members include KChIP1 expressed in brain, KChIP2 expressed in heart, brain, and lung, and KChIP3 (previously identified as calsenilin) expressed in brain and testis. In rat brain, KChIP1 colocalizes with Kv4.3 in granule cells and KChIP2 colocalizes with Kv4.2 in both neocoritcal and subcortical structures. The KChIPs are members of the recoverin/neuronal calcium sensor-1 subfamily of calcium-binding proteins and show 99% nucleotide homology to DREAM, suggesting that KChIPs may have activity beyond modulation of Kv4 channels.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q9NS61

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein (within amino acids 1-270) from the human protein was used as the immunogen for the KChIP2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

KCNIP2/7589

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the KChIP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This KChIP2 antibody is available for research use only.

Storage Conditions

Aliquot the KChIP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the KChIP2 antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

IHC staining of FFPE human cerebellum tissue with KChIP2 antibody (clone KCNIP2/7589) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.