Ki-67 antibody

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Product Specifications

UniProt

P46013

Host

Rabbit

Immunogen

Recombinant human protein (amino acids R5-Q2358) was used as the immunogen for the Ki-67 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, Direct ELISA

Format

Antigen affinity purified

Buffer

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Reconstitution

After reconstitution, the Ki-67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.