Thidiazuron (TDZ)
Thidiazuron (TDZ) Powder is a plant growth regulator and a synthetic diphenylurea cytokinin widely used in plant tissue culture applications to promote shoot regeneration, organogenesis, and somatic embryogenesis.
Product Specifications
Synonyms
1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea; TDZ
HS Code
2934.99
Applications
Used alone or in combination with other hormones for efficient multiplication of in vitro plants.
TDZ can be used in culture media to promote seed germination
Used in agricultural and home gardening.
TDZ is often used in combination with auxin (IAA) in culture media to achieve a balance between shoot and root development.
Assay Protocol
Follow the given steps to prepare the tissue culture media using PCT’s TDZ Powder:
Prepare liquid tissue culture media using PCT’s MS or DKW media, or any other alternative media based on your plant.
Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
Then, add the desired quantity of the plant hormone from the prepared NAA stock solution.
Autoclave the medium for 20 minutes at 15 psi and 121°C.
Your media is ready!
Color
White to Off-White Powder or Crystals
Solubility
Soluble in DMSO, KOH, MeOH
Molecular Formula
C9H8N4OS
Features & Benefits
Easy to use
Effective in stimulating shoot multiplication from explants (tissue samples) in micropropagation
Used to supplement tissue culture media, such as MS media.
Help overcome apical dominance
Crucial for the micropropagation of some plants.
Storage Conditions
2-8°C
Storage Temperature
2-8°C
Shelf Life
3 years from the manufacturing date
Certificate of Analysis
https://cdn.gentaur.com/products/644/5048171235973/coa/tdzpw1.pdf
Product MSDS
https://cdn.gentaur.com/products/644/5048171235973/msds/tdzpw1.pdf
Appearance
White to Off-White Powder or Crystals
Suitability
Suitable for plant cell culture
CAS Number
51707-55-2
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