AF488 azide

AF488 azide is a fluorescent dye that can be used to determine the reactivity of immobilized DIBO groups. It contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. AF488 exhibits the λem and λex wavelength of 520 nm and 470 nm, respectively[1][2].

Product Specifications

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/af488-azide.html

Purity

99.0

Solubility

10 mM in DMSO

Smiles

O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O[K])=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(NCCCN=[N+]=[N-])=O)=C1)O[K]

Molecular Formula

C24H18K2N6O10S2

Molecular Weight

692.76

Precautions

H302, H315, H319, H335

References & Citations

[1]Ruff SM, et al. clickECM: Development of a cell-derived extracellular matrix with azide functionalities. Acta Biomater. 2017 Apr 1;52:159-170.|[2]Chakraborty S, Hu S Y, Wu S H, et al. The interaction affinity between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) analyzed by quantitative FRET[J]. PLoS One, 2015, 10 (3) : e0121399.