ULK1 Antibody

Rabbit Anti-Human ULK1 Polyclonal

Product Specifications

Background

UNC-51-like kinase 1 (ULK1) is a serine/threonine kinase broadly expressed in mammalian tissues, with a critical role in neuronal development and homeostasis. Structurally, ULK1 comprises an N-terminal kinase domain, a central proline/serine-rich region, and a highly conserved C-terminal domain. Functionally, ULK1 is a mammalian homolog of yeast Atg1 and serves as a master regulator of autophagy initiation—a process essential for cellular quality control and survival under stress. In the nervous system, ULK1 has been directly implicated in axonal growth, synaptic remodeling, and neuronal survival. Its ability to interact with multiple autophagy-related (ATG) proteins enables it to orchestrate phosphorylation-dependent signaling cascades that regulate autophagosome formation and intracellular trafficking. Dysregulation of ULK1 activity has been associated with impaired autophagic flux, a hallmark of several neurodegenerative diseases including Alzheimer’s, Parkinson’s, and Huntington’s disease. Given its dual role in autophagy and axonal dynamics, ULK1 represents a promising therapeutic target in neuroscience and neurodegeneration research. Understanding ULK1-mediated signaling pathways offers critical insights into the molecular mechanisms underlying neuronal dysfunction and opens new avenues for the development of autophagy-based interventions in neurodegenerative disorders.

CAS Number

9007-83-4

Specifications

Detects ~100 kDa.

Product Name Alternative

Unc-51 like kinase 1 (C. elegans), Autophagy related protein 1 homolog, Autophagy-related protein 1 homolog, FLJ38455, Serine/threonine-protein kinase ULK1, Unc51.1, ATG1A, UNC51, C. elegans, homolog of, EC:2.7.11.1, Serine/threonine protein kinase Unc51.1, FLJ46475, ATG 1, hATG1, ATG1, Unc-51-like kinase 1, Serine/threonine protein kinase ULK1, ULK1, Unc 51 like kinase 1, KIAA0722, UNC 51, ULK 1, ULK1_HUMAN, UNC51, Unc 51 (C. elegans) like kinase 1, ATG1 autophagy related 1 homolog

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

8408

Swiss Prot

O75385

Accession Number

NP_003556.1

Cellular Locus

Cytoplasm | Cytosol | Preautophagosomal Structure

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)

Target

ULK1

Clonality

Polyclonal

Conjugation

Unconjugated

Tissue Specificity

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Type

Polyclonal

Applications

WB | ICC/IF

Validated Applications

WB,ICC/IF

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12. 2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900. 3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13. 4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5. 5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.