Piccolo Antibody

Rabbit Anti-Human Piccolo Polyclonal

Product Specifications

Background

Piccolo (also known as Aczonin) is a large, multidomain scaffolding protein localized at the presynaptic active zone. It plays a central role in organizing the cytomatrix at active zones (CAZ) and coordinating synaptic vesicle trafficking, exocytosis, and endocytosis. Piccolo interacts with proteins involved in actin dynamics and clathrin-mediated endocytosis, including Abp1 and dynamin, thereby regulating synaptic vesicle recycling. It is often co-expressed with Bassoon, another CAZ protein, to maintain synaptic integrity and plasticity. Disruption of Piccolo expression or function has been linked to neurodevelopmental and neurodegenerative disorders, including schizophrenia and Parkinson’s disease. Its role in synaptic maintenance and plasticity makes Piccolo a promising target for understanding and treating synaptic dysfunction in neurological diseases.

CAS Number

9007-83-4

Specifications

Detects ~550kDa. No cross-reactivity to Bassoon.

Product Name Alternative

Protein piccolo, Aczonin, PCLO, Presynaptic cytomatrix protein, Multidomain presynaptic cytomatrix protein, Piccolo (presynaptic cytomatrix protein), PCLO_HUMAN, ACZ, Pico, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Piccolo mouse homolog of Pico

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

27445

Swiss Prot

Q9Y6V0

Accession Number

NP_055325.2

Cellular Locus

Cell Junction | Synapse

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant Full length human Piccolo Protein, BC001304.1

Target

Piccolo

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | IHC

Validated Applications

WB,IHC

Field of Research

Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers

Purification

Protein A Purified

Detection Range

1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:400) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20 (7) : 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147 (1) : 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281 (9) :6038-6047.