GRP78 Antibody

Rabbit Anti-Rat GRP78 Polyclonal

Product Specifications

Background

GRP78 (also known as BiP) is a 78-kDa glucose-regulated protein and a master regulator of the unfolded protein response (UPR) . As a key ER chaperone, it ensures proper protein folding, assembly, and the clearance of misfolded proteins, especially under stress conditions. Its expression is controlled by an internal ribosome entry site (IRES), which becomes highly active during cellular stress, such as heat shock or oxidative damage. GRP78 is essential for maintaining neuronal homeostasis and preventing apoptosis, making it a critical player in neurodegenerative disease contexts. In neuroscience, GRP78 has demonstrated neuroprotective effects against glutamate toxicity and oxidative stress. Its levels are notably reduced in the brains of Alzheimer’s disease patients, highlighting its potential as a biomarker and therapeutic target. Additionally, GRP78 supports embryonic development and pluripotent cell survival, further underscoring its importance in neural cell viability. However, its overexpression under extreme stress may contribute to drug resistance in neuro-oncology, adding complexity to its therapeutic modulation.

CAS Number

9007-83-4

Specifications

Detects ~78kDa.

Product Name Alternative

GRP78, GRP-78, Grp78, GRP 78, HSPA5, HSPA 5, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, BiP, BIP, Binding immunoglobulin protein, Immunoglobulin heavy chain-binding protein, Immunoglobulin Heavy Chain Binding Protein, Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78, Endoplasmic reticulum lumenal, Ca2+ binding protein grp78, Glucose Regulated Protein 78kDa, 78 kDa glucose-regulated protein, 78 kDa glucose regulated protein, HSCe70, MIF2, mBiP, Sez7, AL022860, AU019543, D2Wsu141e, D2Wsu17e, FLJ26106, GRP78_HUMAN

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

25617

Swiss Prot

P06761

Accession Number

NP_037215.1

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome | Cytoplasm

Host

Rabbit

Species Reactivity

Human,Mouse,Rat,Bovine,Fungi,Hamster,Monkey,Rabbit,Xenopus,Dog

Immunogen

Rat GRP78 (Bip) synthetic peptide conjugated to KLH

Target

GRP78

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal

Applications

WB | IHC | ICC/IF | IP

Validated Applications

WB,IHC,ICC/IF,IP

Field of Research

Purification

Peptide Affinity Purified

Detection Range

A 1:1000 dilution of SPC-107 was sufficient for detection of Grp78 in 10 µg of rat tissue lysate by ECL immunoblot analysis.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Cho, S. et al. (2007) . Mol Cell Biol 27 (1) : 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15) : 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19:4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65 (13) : 5785-91.