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Sulfotyrosine Antibody: HRP

Mouse Anti-Sulfotyrosine Monoclonal IgG1

Product Specifications

Background

Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.

Specifications

Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Product Name Alternative

Sulfotyrosine Antibody, Tyrosine sulfation Antibody, Sulfated Tyrosine Antibody, S-Tyrosine Antibody, sTYR Antibody, Sulphotyrosine Antibody, Tyrosine Sulphation Antibody, Sulphated Tyrosine Antibody

Synonyms

Sulfotyrosine Antibody, Clone 12B11: HRP

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Target

Sulfotyrosine

Clonality

Monoclonal

Isotype

IgG1

Clone

12B11 (Formerly sold as 7C5)

Conjugation

HRP

Validated Applications

WB,IHC,ELISA

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-522 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Lee, R.W., Huttner W.B. (1983). J Biol Chem. 258(18):11326-34.

Product Datasheet

https://cdn.gentaur.com/products/400/4293489/datasheet/smc-522d-hrp.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293489/msds/smc-522d-hrp.pdf

CAS Number

9007-83-4

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