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7-Ketocholesterol Antibody: RPE

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b

Product Specifications

Background

Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.

Specifications

Specific for 7-ketocholesterol modified proteins.

Product Name Alternative

7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody

Synonyms

7-Ketocholesterol Antibody, Clone 7E1: RPE

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Target

7-Ketocholesterol

Clonality

Monoclonal

Isotype

IgG2b

Clone

7E1

Conjugation

RPE

Validated Applications

WB,IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-512 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Product Datasheet

https://cdn.gentaur.com/products/400/4293242/datasheet/smc-512d-rpe.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293242/msds/smc-512d-rpe.pdf

CAS Number

9007-83-4

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