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7-Ketocholesterol Antibody: Biotin

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b

Product Specifications

Background

Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.

Specifications

Specific for 7-ketocholesterol modified proteins.

Product Name Alternative

7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody

Synonyms

7-Ketocholesterol Antibody, Clone 7E1: Biotin

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Target

7-Ketocholesterol

Clonality

Monoclonal

Isotype

IgG2b

Clone

7E1

Conjugation

Biotin

Validated Applications

WB,IHC,ICC/IF |ELISA

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-512 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Product Datasheet

https://cdn.gentaur.com/products/400/4293232/datasheet/smc-512d-bi.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293232/msds/smc-512d-bi.pdf

CAS Number

9007-83-4

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