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4-Hydroxy-2-hexenal Antibody: ATTO 390

Mouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b

Product Specifications

Background

Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.

Specifications

Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Product Name Alternative

4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody

Synonyms

4-Hydroxy-2-hexenal Antibody, Clone 6F10: ATTO 390

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).

Target

4-Hydroxy-2-hexenal

Clonality

Monoclonal

Isotype

IgG2b

Clone

6F10

Conjugation

ATTO 390

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-510 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.

Product Datasheet

https://cdn.gentaur.com/products/400/4293171/datasheet/smc-510d-a390.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293171/msds/smc-510d-a390.pdf

CAS Number

9007-83-4

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