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Hexanoyl-Lysine adduct Antibody: ATTO 488

Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1

Product Specifications

Background

Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.

Specifications

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Product Name Alternative

Hexanoyl-Lysine adduct Antibody, HEL (Hexanoyl-Lysine adduct) Antibody, HEL Antibody, HEL Adduct Antibody, Hexanoyl-Lys adduct Antibody, Hexanoyl-Lys Antibody, Hexanoyl-Lysine (HEL) adduct Antibody, Hexanoyl-Lys (HEL) Antibody, Hexanoyl Lysine adduct Antibody, Hexanoyl-Lysine adduct-modified protein Antibody, Hexanoyl-Lysine Adduct (HEL) Antibody

Synonyms

Hexanoyl-Lysine adduct Antibody, Clone 5E8: ATTO 488

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

Target

Hexanoyl-Lysine adduct

Clonality

Monoclonal

Isotype

IgG1

Clone

5E8

Conjugation

ATTO 488

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-509 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.

Product Datasheet

https://cdn.gentaur.com/products/400/4293147/datasheet/smc-509d-a488.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293147/msds/smc-509d-a488.pdf

CAS Number

9007-83-4

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