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Hexanoyl-Lysine adduct Antibody: APC

Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1

Product Specifications

Background

Hexanoyl-lysine adduct (HEL) is a lysine adduct of 13-HPODE and is produced by the oxidation of omega-6 polyunsaturated fatty acids (1). It is a biomarker for the initial stage of lipid peroxidation. Lipid peroxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging.

Specifications

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.

Product Name Alternative

Hexanoyl-Lysine adduct Antibody, HEL (Hexanoyl-Lysine adduct) Antibody, HEL Antibody, HEL Adduct Antibody, Hexanoyl-Lys adduct Antibody, Hexanoyl-Lys Antibody, Hexanoyl-Lysine (HEL) adduct Antibody, Hexanoyl-Lys (HEL) Antibody, Hexanoyl Lysine adduct Antibody, Hexanoyl-Lysine adduct-modified protein Antibody, Hexanoyl-Lysine Adduct (HEL) Antibody

Synonyms

Hexanoyl-Lysine adduct Antibody, Clone 5D9: APC

UNSPSC

12352203

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

Target

Hexanoyl-Lysine adduct

Clonality

Monoclonal

Isotype

IgG1

Clone

5D9

Conjugation

APC

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-508 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); IHC (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sakai, K et al. (2014) Subcell. Biochem. 77:61-72.

Product Datasheet

https://cdn.gentaur.com/products/400/4293130/datasheet/smc-508d-apc.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4293130/msds/smc-508d-apc.pdf

CAS Number

9007-83-4

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