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Neuroligin 1 Antibody: HRP

Mouse Anti-Rat Neuroligin 1 Monoclonal IgG1

Product Specifications

Background

Neuroligin-1 is a neuronal cell surface protein belonging to the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses for their normal, functional development, and is necessary to the regulation of synaptic plasticity and the development of long-term memory within the adult amygdala in mammals. It is believed to participate in cell-cell-interaction through the assembly of intracellular junction by the binding of beta-neurexins, and may also be a factor in the maintenance and assembly of synaptic junctions. It is also thought to have involvement in excitatory synaptic specification. Within brain tissue, Neuroligin-1 is primarily observed in neurons and spinal cord.

Specifications

Detects ~120kDa. Does not cross-react with other Neuroligins.

Product Name Alternative

NLG 1 Antibody, KIAA1070 Antibody, MGC45115 Antibody, Neuroligin-1 Antibody, NL1 Antibody, NLG1 Antibody, Nlgn1 Antibody

Synonyms

Neuroligin 1 Antibody, Clone N97A/31: HRP

UNSPSC

12352203

Gene ID

116647

Swiss Prot

Q62765

Accession Number

NP_446320.1.

Cellular Locus

Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) >40% identity with Neuroligin-2 and -3.

Target

Neuroligin 1

Clonality

Monoclonal

Isotype

IgG1

Clone

N97A/31 (Formerly sold as S97A-31)

Conjugation

HRP

Validated Applications

WB,IHC,ELISA

Purification

Protein G Purified

Limit Of Detection

2 µg/ml of SMC-463 was sufficient for detection of Neuroligin-1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Product Datasheet

https://cdn.gentaur.com/products/400/4292114/datasheet/smc-463d-hrp.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4292114/msds/smc-463d-hrp.pdf

CAS Number

9007-83-4

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