LGI1 Antibody: APC
Mouse Anti-Mouse LGI1 Monoclonal IgG2a
Product Specifications
Background
The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.
Specifications
Detects ~60kDa.
Product Name Alternative
ADLTE Antibody, ADPAEF Antibody, ADPEAF Antibody, Epitempin 1 Antibody, EPT Antibody, ETL1 Antibody, IB1099 Antibody, leucine rich glioma inactivated 1 Antibody, OTTHUMP00000020121 Antibody, OTTHUMP00000020122
Antibody
Synonyms
LGI1 Antibody, Clone N283/7: APC
UNSPSC
12352203
Gene ID
56839
Swiss Prot
Q9JIA1.1
Accession Number
NP_064674.1
Cellular Locus
Cell Junction | Golgi Apparatus | Endoplasmic Reticulum
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
Target
LGI1
Clonality
Monoclonal
Isotype
IgG2a
Clone
N283/7 (Formerly sold as S283-7)
Conjugation
APC
Validated Applications
IHC,ICC/IF
Purification
Protein G Purified
Limit Of Detection
1 µg/ml of SMC-461 was sufficient for detection of LGI1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000); optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
95.64mM Phosphate, 2.48mM MES and 2mM EDTA
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Chernova O.B., Somerville R.P. and Cowell J.K. (1998) Oncogene. 17:2873-81.
2. Fialka F., et al. (2008) Oral Oncol.
3. Gu W., et al. (2005) Mol. Biol. Evol. 22:2209-16.
4. Fukata Y., et al. (2006) Science. 313:1792-5.
Product Datasheet
https://cdn.gentaur.com/products/400/4292055/datasheet/smc-461d-apc.pdf
Product MSDS
https://cdn.gentaur.com/products/400/4292055/msds/smc-461d-apc.pdf
CAS Number
9007-83-4
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