GFAP Antibody: Biotin

Mouse Anti-Human GFAP Monoclonal IgG1

Product Specifications

Background

The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia.

Specifications

Detects ~50kDa. Cross-reacts with GFAP-R416W and other GFAP mutant proteins.

Product Name Alternative

Glial fibrillary acidic protein Antibody, Intermediate filament protein Antibody, Astrocyte Antibody, gfapl Antibody, DKFZp459C0729 Antibody, MGC139638 Antibody, FLJ45472 Antibody, AI836096 Antibody, GFAP Antibody

Synonyms

GFAP Antibody, Clone N206A/8: Biotin

UNSPSC

12352203

Gene ID

2670

Swiss Prot

P14136

Accession Number

NP_001124491.1

Cellular Locus

Cytoplasm

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse

Target

GFAP

Clonality

Monoclonal

Isotype

IgG1

Clone

N206A/8 (Formerly sold as S206A-8)

Conjugation

Biotin

Validated Applications

WB,IHC,ICC/IF |ELISA

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-441 was sufficient for detection of GFAP in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Velasco M.E., et al. (1980) Cancer. 45:484. 2. Bonnin J.M., et al. (1984) Acta Neuropathology. 62:185. 3. Lee VM-Y., et al. (1984) J. Neurochem. 42:25-32 (1984). 4. Trojanowski JQ et al. (1986) J. Neurochem. 6(3): 650-660 (1986). 5. Schmidt ML et al; Lab Invest 56:282-294 (1987). 6. Kosik KS et al; Neuron 1:817-825 (1988). 7. Schmidt ML et al; Lab Invest 59:460-466 (1988). 8. Mokuna, K, et al: J Neurosci Res 23:396 (1989). 9. Molenaar, et al; Exp Neurology 108:1-9 (1990). 10. Tohyama T et al; Am J Pathol, 142:871-882 (1993). 11. Tohyama T et al; Am J Pathol 142:883-892 (1993). 12. Thilenius, A.R.B., et al; J. Immunol. 162(2): 643-650 (1999).

Product Datasheet

https://cdn.gentaur.com/products/400/4291607/datasheet/smc-441d-bi.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4291607/msds/smc-441d-bi.pdf

CAS Number

9007-83-4

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