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Notch1 Antibody: PerCP

Mouse Anti-Mouse Notch1 Monoclonal IgG1

Product Specifications

Background

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Specifications

Detects >270kDa. Does not cross-react with Notch 2 or Notch3.

Product Name Alternative

Neurogenic locus notch homolog protein 1 Antibody, hN1 Antibody, Translocation associated notch protein Antibody, TAN1 Antibody, TAN-1 Antibody, Motch A Antibody, mT14 Antibody, p300 Antibody, Motch Antibody, Mis6 Antibody, Neurogenic locus notch protein homolog Antibody, NICD Antibody, Notch 1 intracellular domain Antibody, Notch gene homolog 1 (Drosophila) Antibody, Notch homolog 1 Antibody, translocation-associated (Drosophila) Antibody, NOTCH Drosophila Antibody, homolog of 1 Antibody, Translocation associated notch homolog Antibody, Translocation associated notch protein TAN 1 Antibody, xotch Antibody

Synonyms

Notch1 Antibody, Clone N253/32: PerCP

UNSPSC

12352203

Gene ID

18128

Swiss Prot

Q01705

Accession Number

NP_032740.3

Cellular Locus

Cell Membrane | Nucleus

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1

Target

Notch1

Clonality

Monoclonal

Isotype

IgG1

Clone

N253/32 (Formerly sold as S253-32)

Conjugation

PerCP

Validated Applications

WB,IHC,ICC/IF,ELISA

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719. 2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company. 3. Lindsell C.E., et al. (1995) Cell 80: 909-917. 4. Uyttendaele H., et al. (1996) Development 122: 2251-2259. 5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.

Product Datasheet

https://cdn.gentaur.com/products/400/4291341/datasheet/smc-430d-pcp.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4291341/msds/smc-430d-pcp.pdf

CAS Number

9007-83-4

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