ASIC1 Antibody: ATTO 488

Mouse Anti-Mouse ASIC1 Monoclonal IgG1

Product Specifications

Background

Acid sensing ion channel ASIC1, also designated ACCN2, BNAC2 and ASIC1a, is present in brain as a 4.3-kb transcript with localization to rat dorsal root ganglia. Insitu hybridization of rat brain suggests that ASIC1 is most abundant in the main olfactory bulb, cerebral cortex, hippocampal formation, habenula, basolateral amygdaloid nuclei and cerebellum. ASIC1 and H+-gated currents may contribute to the development of fear and anxiety.

Specifications

Detects ~60kDa.

Product Name Alternative

ACCN2 Antibody, Acid-sensing (proton-gated) ion channel Antibody, Acid-sensing ion channel 1 Antibody, Amiloride-sensitive cation channel 2 neuronal Antibody, BNAC2 Antibody, Brain sodium channel 2 Antibody

Synonyms

ASIC1 Antibody, Clone N271/44: ATTO 488

UNSPSC

12352203

Gene ID

11419

Swiss Prot

Q6NXK8

Accession Number

NP_033727.1

Cellular Locus

Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of mouse ASIC1

Target

ASIC1

Clonality

Monoclonal

Isotype

IgG1

Clone

N271/44 (Formerly sold as S271-44)

Conjugation

ATTO 488

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-427 was sufficient for detection of ASIC1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Garcia-Anoveros J., Derfler B., Neville-Golden J., Hyman B.T. and Corey D.P. (1997) Proc. Natl. Acad. Sci. USA 94: 1459-1464. 2. Waldmann R., Champigny G., Bassilana F., Heurteaux C. and Lazdunski M. (1997) Nature. 386: 173-177. 3. Grunder S., Geisler H.S., Rainier S. and Fink J.K. (2001) Eur. J. Hum. Genet. 9:672-676. 4. Chen C.C., Zimmer A., Sun W.H., Hall J., Brownstein M.J. and Zimmer A. (2002) Proc. Natl. Acad. Sci. USA 99: 8992-8997. 5. Wemmie J.A., et al. (2004) Proc. Natl. Acad. Sci. USA 101: 3621-3626 .

Product Datasheet

https://cdn.gentaur.com/products/400/4291247/datasheet/smc-427d-a488.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4291247/msds/smc-427d-a488.pdf

CAS Number

9007-83-4

Curated Selection

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View Details

MBS6286744-01 0.2 mL

CLEC9A, ID (CLEC9A, C-type lectin domain family 9 member A) (PE)

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