Dicer Antibody
Mouse Anti-Mouse Dicer Monoclonal IgG1
Product Specifications
Background
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
Specifications
Detects ~215kDa.
Product Name Alternative
Dicer1 Antibody, DCR Antibody, DCR1 Antibody, DCR-1 Antibody, Double-strand-specific ribonuclease Antibody, Helicase with RNase motif Antibody, HERNA Antibody, Helicase MOI Antibody, KIAA0928 Antibody, Endoribonuclease Dicer Antibody
Synonyms
Dicer Antibody, Clone N167/7
UNSPSC
12352203
Gene ID
192119
Swiss Prot
Q8R418
Accession Number
NP_683750.2
Cellular Locus
Cytoplasm
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Target
Dicer
Clonality
Monoclonal
Isotype
IgG1
Clone
N167/7 (Formerly sold as S167-7)
Conjugation
Unconjugated
Validated Applications
WB,ICC/IF
Purification
Protein G Purified
Limit Of Detection
1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Weight
0.1
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.
2. Lee Y., et al. (2003) Nature 425: 415-9.
3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
Product MSDS
https://cdn.gentaur.com/products/400/4290970/msds/smc-416d.pdf
CAS Number
9007-83-4
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