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Clcn3 Antibody: ATTO 390

Mouse Anti-Rat Clcn3 Monoclonal IgG1

Product Specifications

Background

Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.

Specifications

Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results).

Product Name Alternative

CIC3 Antibody, CLC3 Antibody, CLCN 3 Antibody, Chloride channel 3 Antibody, Chloride channel protein 3 Antibody, Chloride channel Antibody, voltage-sensitive 3 Antibody, Chloride transporter ClC 3 Antibody, DKFZp564I0463 Antibody, H(+)/Cl(-) exchange transporter 3 Antibody, Chloride transporter ClC-3 Antibody, ClC-3 Antibody, H(+)/Cl(-) exchange transporter 3 Antibody

Synonyms

ClCN3 Antibody, Clone N258/5: ATTO 390

UNSPSC

12352203

Gene ID

84360

Swiss Prot

P51792

Accession Number

NP_445815.2

Cellular Locus

Membrane | Endosome | Endosome membrane | Cytoplasmic Vesicle | Secretory Vesicle Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 98-115 (cytoplasmic N-terminus) of rat Clcn3

Target

ClCN3

Clonality

Monoclonal

Isotype

IgG1

Clone

N258/5 (Formerly sold as S258-5)

Conjugation

ATTO 390

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-408 was sufficient for detection of Clcn3 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1.  www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true

Product Datasheet

https://cdn.gentaur.com/products/400/4290771/datasheet/smc-408d-a390.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290771/msds/smc-408d-a390.pdf

CAS Number

9007-83-4

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