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mGluR1/5 Antibody: ATTO 488

Mouse Anti-Rat mGluR1/5 Monoclonal IgG1

Product Specifications

Background

The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1,2).

Specifications

Detects ~130kDa (both mGluR1 and mGluR5).

Product Name Alternative

Glutamate receptor metabotropic 5,GPRC1E Antibody, GRM 5 Antibody, GRM5 Antibody, Metabotropic glutamate receptor 5 Antibody, Metabotropic glutamate receptor 5 variant F Antibody, Metabotropic glutamate receptor 5 variant G Antibody, Metabotropic glutamate receptor 5 variant H Antibody, mGlu5 Antibody, mGluR5 Antibody, mGluR5a Antibody, mGluR5b Antibody, Glutamate receptor metabotropic 1 Antibody, glutamate receptor Antibody, metabotropic 1 Antibody, GPRC1A Antibody, GRM 1 Antibody, GRM1 alpha Antibody, GRM1 Antibody, GRM1_HUMAN Antibody, GRM1A Antibody, Metabotropic glutamate receptor 1 Antibody, mGlu1 Antibody, mGluR1 alpha Antibody, mGluR1 Antibody, mGluR1A Antibody

Synonyms

mGluR1/5 Antibody, Clone N75/33: ATTO 488

UNSPSC

12352203

Gene ID

24418

Swiss Prot

P31424

Accession Number

NP_058708.1

Cellular Locus

Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Target

mGluR5

Clonality

Monoclonal

Isotype

IgG1

Clone

N75/33 (Formerly sold as S75-33)

Conjugation

ATTO 488

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-407 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Mayer M.L. (2005) Current Opinion in Neurobio. 15(3): 282-288. 2. Keinane K., et al. (1990) Science. 249: 556-560.

Product Datasheet

https://cdn.gentaur.com/products/400/4290747/datasheet/smc-407d-a488.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290747/msds/smc-407d-a488.pdf

CAS Number

9007-83-4

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