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Ankyrin G Antibody: RPE

Mouse Anti-Human Ankyrin G Monoclonal IgG1

Product Specifications

Background

Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.

Specifications

Detects >~200kDa.

Product Name Alternative

ANK-3 Antibody, ANK3 Antibody, ankyrin 3 Antibody, Ankryin G119 Antibody, brain specific ankyrin G Antibody, CHANK3 Antibody, ankyrin 3 (G) Antibody, Ankyrin-3 Antibody, Ankyrin-G Antibody, node of Ranvier (ankyrin G) Antibody, Ankyrin 3 Antibody, node of Ranvier Antibody, Ankyrin G119 Antibody, FLJ45464 Antibody, OTTHUMP00000217458 Antibody, OTTHUMP00000217575 Antibody, RP11 369L1.1 Antibody

Synonyms

Ankyrin G Antibody, Clone N106/20: RPE

UNSPSC

12352203

Gene ID

288

Swiss Prot

Q12955

Accession Number

NP_066267.2

Cellular Locus

Cytoplasm | Cytoskeleton | Cell Projection | Axon | Cell Membrane | Sarcolemma | Cell Junction | Synapse | Postsynaptic Cell Membrane | Lysosome

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein 1000 C-terminal amino acids of human Ankyrin G (also known as ANK-3 or ankyrin-3) encompassing all of Ankyrin G with the exception of Ankyrin repeats

Target

Ankyrin G

Clonality

Monoclonal

Isotype

IgG1

Clone

N106/20 (Formerly sold as S106-20)

Conjugation

RPE

Validated Applications

WB,IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-404 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.

Product Datasheet

https://cdn.gentaur.com/products/400/4290692/datasheet/smc-404d-rpe.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290692/msds/smc-404d-rpe.pdf

CAS Number

9007-83-4

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