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Ankyrin B Antibody: ATTO 390

Mouse Anti-Human Ankyrin B Monoclonal IgG1

Product Specifications

Background

Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.

Specifications

Detects ~200kDa. No cross reactivity against Ankyrin-G.

Product Name Alternative

ANK-2 Antibody, ANK2 Antibody, Ankyrin 2 Antibody, Ankyrin-2 Antibody, Ankyrin nonerythroid Antibody, Brain ankyrin Antibody, non-erythroid ankyrin Antibody, Ankyrin-B Antibody

Synonyms

Ankyrin B Antibody, Clone N105/13: ATTO 390

UNSPSC

12352203

Gene ID

287

Swiss Prot

Q01484

Accession Number

NP_001120965.1

Cellular Locus

Cytoplasm | Cytoskeleton | Myofibril | Sarcomere | M line | Apical Cell Membrane | Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthetic peptide amino acids 203-496 of human Ankyrin-B

Target

Ankyrin B

Clonality

Monoclonal

Isotype

IgG1

Clone

N105/13 (Formerly sold as S105-13)

Conjugation

ATTO 390

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-400 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.

Product Datasheet

https://cdn.gentaur.com/products/400/4290571/datasheet/smc-400d-a390.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290571/msds/smc-400d-a390.pdf

CAS Number

9007-83-4

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