MMP9 Antibody
Mouse Anti-Rat MMP9 Monoclonal IgG2a
Product Specifications
Background
MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
Specifications
Detecs ~92kDa and ~82kDa (pro and active forms).
Product Name Alternative
MMP-9 Antibody, CLG4B Antibody, 82kDa matrix metalloproteinase-9 Antibody, collagenease type 4 beta Antibody, GELB Antibody, Macrophage gelatinase Antibody, MANDP2 Antibody, Type V collagenase Antibody, 92 kDa gelatinase Antibody, 92 kDa type IV collagenase Antibody, Gelatinase B Antibody, MMP9 Antibody, Matrix metalloproteinase 9 Antibody
Synonyms
MMP9 Antibody, Clone L51/82
UNSPSC
12352203
Gene ID
81687
Swiss Prot
P50282
Accession Number
NP_112317.1
Cellular Locus
Extracellular Matrix
Host
Mouse
Species Reactivity
Human,Mouse,Rat
Immunogen
Fusion protein amino acids 1-708 (full length) of rat MMP9
Target
MMP9
Clonality
Monoclonal
Isotype
IgG2a
Clone
L51/82 (Formerly sold as S51-82)
Conjugation
Unconjugated
Validated Applications
WB,IHC,ICC/IF,IP,FCM
Purification
Protein G Purified
Limit Of Detection
1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129.
2. Coussens L.M., et al. (2002) Science 295: 2387-2391.
3. Sternlicht M.D., et al. (1999) Cell 98: 137-146.
4. Vu T.H., et al. (1998) Cell 93: 411-422.
5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.
Product MSDS
https://cdn.gentaur.com/products/400/4290494/msds/smc-396s.pdf
CAS Number
9007-83-4
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