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VGLUT2 Antibody: APC

Mouse Anti-Rat VGLUT2 Monoclonal IgG1

Product Specifications

Background

The ATP-dependent, chloride-sensitive vesicular glutamate transporters (VGLUT) include BNPI (VGLUT1), VGLUT2 (DNPI) and VGLUT3. The brain expresses BNPI (brain specific Na+-dependent inorganic phosphate (Pi) cotransporter) and VGLUT2 in a complementary fashion. The telencephalic regions express BNPI, whereas the lower brainstem and diencephalic regions express VLGUT2. Rat pinealocytes express both BNPI and VGLUT2. The striatum, hippocampus, cerebral cortex and raphe nuclei express VGLUT3 in a small number of neurons. Pancreatic α and β cells express BNPI and VGLUT2 in response to glucose concentrations. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.

Specifications

Detects ~60kDa.

Product Name Alternative

Differentiation associated BNPI Antibody, DNPI Antibody, SLC17A6 Antibody, Solute carrier family 17 member 6 Antibody, Vesicular glutamate transporter 2 Antibody, Differentiation associated Na dependent inorganic phosphate cotransporter Antibody, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 Antibody, Differentiation associated Na(+) dependent inorganic phosphate cotransporter Antibody, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter Antibody, Sodium dependent inorganic phosphate cotransporter Antibody

Synonyms

VGLUT2 Antibody, Clone N29/29: APC

UNSPSC

12352203

Gene ID

84487

Swiss Prot

Q9JI12

Accession Number

NP_445879.1

Cellular Locus

Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

Target

VGLUT2

Clonality

Monoclonal

Isotype

IgG1

Clone

N29/29 (Formerly sold as S29-29)

Conjugation

APC

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-395 was sufficient for detection of VGLut2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Aihara Y., et al. (2000) J Neurochem. 74: 2622-2625. 2. Kaneko T., and Fujiyama F. (2002) Neurosci Res. 42: 243-250. 3. Gras C., et al. (2002) J Neurosci. 22: 5442-5451. 4. Takamori S., Malherbe P., Broger C., and Jahn R. (2002) EMBO Rep. 3: 798-803. 5. Morimoto R., Hayashi M., Yatsushiro S., Otsuka M., Yamamoto A., and Moriyama Y. (2003) J Neurochem. 84: 382-391. 6. Bai L., Zhang X., and Ghishan F.K. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 284: G808-814.

Product Datasheet

https://cdn.gentaur.com/products/400/4290455/datasheet/smc-395d-apc.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290455/msds/smc-395d-apc.pdf

CAS Number

9007-83-4

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