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VGLUT1 Antibody: RPE

Mouse Anti-Rat VGLUT1 Monoclonal IgG1

Product Specifications

Background

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Specifications

Detects ~52kDa. No cross-reactivity against VGlut2.

Product Name Alternative

BNPI Antibody, SLC17A1 Antibody, SLC17A1/VGLUT1 Antibody, Solute Carrier family 17 member 7 Antibody, Vesicular glutamate transporter 1 Antibody, VGLUT 1 Antibody, Brain-specific Na(+)-dependent inorganic phosphate cotransporter Antibody

Synonyms

VGLUT1 Antibody, Clone N28/9: RPE

UNSPSC

12352203

Gene ID

116638

Swiss Prot

Q62634

Accession Number

NP_446311.1

Cellular Locus

Cytoplasmic Vesicle | Secretory Vesicle | Synaptic Vesicle Membrane | Membrane | Cell Junction | Synapse

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Target

VGLUT1

Clonality

Monoclonal

Isotype

IgG1

Clone

N28/9 (Formerly sold as S28-9)

Conjugation

RPE

Validated Applications

WB,IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163. 2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.

Product Datasheet

https://cdn.gentaur.com/products/400/4290442/datasheet/smc-394d-rpe.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290442/msds/smc-394d-rpe.pdf

CAS Number

9007-83-4

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