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GABA-A Receptor Alpha-1 Antibody: ATTO 488

Mouse Anti-Human GABA-A Receptor Alpha-1 Monoclonal IgG2A

Product Specifications

Background

The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).

Specifications

Detects ~55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or –Alpha3.

Product Name Alternative

ECA4 antibody, EJM antibody, EJM5 antibody, Gaba receptor alpha 1 polypeptide antibody, GABA(A) receptor antibody, GABA(A) receptor subunit alpha 1 antibody, GABA(A) receptor subunit alpha-1 antibody, GABA(A) receptor, alpha 1 antibody, GABRA 1 antibody, GABRA1 antibody, Gamma aminobutyric acid (GABA) A receptor alpha 1 antibody, Gamma aminobutyric acid A receptor alpha 1 antibody, Gamma aminobutyric acid receptor subunit alpha 1 antibody, Gamma-aminobutyric acid receptor subunit alpha-1 antibody, GBRA1_HUMAN antibody

Synonyms

GABA A Receptor Alpha-1 Antibody, Clone N95/35: ATTO 488

UNSPSC

12352203

Gene ID

14394

Swiss Prot

P14867

Accession Number

NP_034380.1

Cellular Locus

Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1

Target

GABA A Receptor

Clonality

Monoclonal

Isotype

IgG2A

Clone

N95/35 (Formerly sold as S95-35)

Conjugation

ATTO 488

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-346 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.

Product Datasheet

https://cdn.gentaur.com/products/400/4290272/datasheet/smc-346d-a488.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4290272/msds/smc-346d-a488.pdf

CAS Number

9007-83-4

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