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HCN4 Antibody: ATTO 390

Mouse Anti-Rat HCN4 Monoclonal IgG1

Product Specifications

Background

Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (1).

Specifications

Detects ~130kDa. No cross-reactivity against other HCNs.

Product Name Alternative

Hyperpolarization activated cyclic nucleotide gated potassium channel 4 Antibody, HCN 4 Antibody, Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 4 Antibody

Synonyms

HCN4 Antibody, Clone N114/10: ATTO 390

UNSPSC

12352203

Gene ID

59266

Swiss Prot

Q9JKA7

Accession Number

NP_067690.1

Cellular Locus

Membrane

Host

Mouse

Species Reactivity

Human,Mouse,Rat,Rabbit

Immunogen

Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4

Target

HCN4

Clonality

Monoclonal

Isotype

IgG1

Clone

N114/10 (Formerly sold as S114-10)

Conjugation

ATTO 390

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-320 was sufficient for detection of HCN4 in 10 µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34233

Product Datasheet

https://cdn.gentaur.com/products/400/4289671/datasheet/smc-320d-a390.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4289671/msds/smc-320d-a390.pdf

CAS Number

9007-83-4

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