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N-terminal Arginylation Antibody: RPE

Mouse Anti-N-terminal Arginylation Monoclonal IgG3

Product Specifications

Background

Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).

Specifications

Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine.

Product Name Alternative

N-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody

Synonyms

N-terminal Arginylation Antibody, Clone 11G1: RPE

UNSPSC

12352203

Cellular Locus

Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic N-terminal arginylated peptide conjugated to KLH

Target

N-terminal Arginylation

Clonality

Monoclonal

Isotype

IgG3

Clone

11G1

Conjugation

RPE

Validated Applications

WB,IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-265 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

Product Datasheet

https://cdn.gentaur.com/products/400/4289067/datasheet/smc-265d-rpe.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4289067/msds/smc-265d-rpe.pdf

CAS Number

9007-83-4

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