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N-terminal Arginylation Antibody: HRP

Mouse Anti-N-terminal Arginylation Monoclonal IgG1

Product Specifications

Background

Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).

Specifications

Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Product Name Alternative

N-terminal Arginine Antibody, N-terminal Arginylation Antibody, N-terminal Arginylated Antibody, N terminal Arginine Antibody, N terminal Arginylation Antibody, N terminal Arginylated Antibody, Amino-terminal Arginine Antibody, Amino-terminal Arginylation Antibody, Amino-terminal Arginylated Antibody, Amino terminal Arginine Antibody, Amino terminal Arginylation Antibody, Amino terminal Arginylated Antibody

Synonyms

N-terminal Arginylation Antibody, Clone 4A9: HRP

UNSPSC

12352203

Cellular Locus

Endoplasmic Reticulum

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Synthetic N-terminal arginylated peptide conjugated to KLH

Target

N-terminal Arginylation

Clonality

Monoclonal

Isotype

IgG1

Clone

4A9

Conjugation

HRP

Validated Applications

WB,IHC,ELISA

Purification

Protein G Purified

Limit Of Detection

A 1:1000 dilution of SMC-263 was sufficient for detection of N-terminal Arginylation in 0.5 ug of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000); FCM (1:50); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.

Product Datasheet

https://cdn.gentaur.com/products/400/4289014/datasheet/smc-263d-hrp.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4289014/msds/smc-263d-hrp.pdf

CAS Number

9007-83-4

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