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Ubiquitin Antibody: ATTO 594

Mouse Anti-Human Ubiquitin Monoclonal IgG1

Product Specifications

Background

Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Specifications

Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.

Product Name Alternative

Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody

Synonyms

Ubiquitin Antibody, Clone FK2: ATTO 594

UNSPSC

12352203

Gene ID

7314

Swiss Prot

P0CG47

Accession Number

BAC56955.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Human Ubiquitin conjugated lysozyme

Target

Ubiquitin

Clonality

Monoclonal

Isotype

IgG1

Clone

FK2

Conjugation

ATTO 594

Validated Applications

IHC,ICC/IF

Purification

Protein A Purified

Limit Of Detection

A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:10000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

Product Datasheet

https://cdn.gentaur.com/products/400/4288338/datasheet/smc-214d-a594.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4288338/msds/smc-214d-a594.pdf

CAS Number

9007-83-4

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