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Ubiquitin Antibody: APC

Mouse Anti-Human Ubiquitin Monoclonal IgM

Product Specifications

Background

Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

Specifications

Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

Product Name Alternative

Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody

Synonyms

Ubiquitin Antibody, Clone FK1: APC

UNSPSC

12352203

Gene ID

7314

Swiss Prot

P0CG47

Accession Number

BAC56955.1

Cellular Locus

Cytoplasm | Nucleus

Host

Mouse

Species Reactivity

Species Independent

Immunogen

Human Ubiquitin conjugated lysozyme

Target

Ubiquitin

Clonality

Monoclonal

Isotype

IgM

Clone

FK1

Conjugation

APC

Validated Applications

IHC,ICC/IF

Purification

Gel filtration

Limit Of Detection

A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:10000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

95.64mM Phosphate, 2.48mM MES and 2mM EDTA

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.

Product Datasheet

https://cdn.gentaur.com/products/400/4288320/datasheet/smc-213d-apc.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4288320/msds/smc-213d-apc.pdf

CAS Number

9007-83-4

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