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EndoPDI Antibody: ATTO 488

Mouse Anti-Human EndoPDI Monoclonal IgG2a

Product Specifications

Background

Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).

Specifications

Detects ~48kDa.

Product Name Alternative

Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras Antibody

Synonyms

EndoPDI Antibody, Clone 2E7/7: ATTO 488

UNSPSC

12352203

Gene ID

81567

Swiss Prot

Q8NBS9

Accession Number

NP_001139021

Cellular Locus

Endoplasmic Reticulum | Endoplasmic Reticulum Lumen

Host

Mouse

Species Reactivity

Human

Immunogen

Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the Human EndoPDI protein

Target

PDI

Clonality

Monoclonal

Isotype

IgG2a

Clone

2E7/7

Conjugation

ATTO 488

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:200); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.

Product Datasheet

https://cdn.gentaur.com/products/400/4288138/datasheet/smc-204d-a488.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4288138/msds/smc-204d-a488.pdf

CAS Number

9007-83-4

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