HSP47 Antibody
Mouse Anti-Human HSP47 Monoclonal IgG1 Kappa
Product Specifications
Background
HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of HSP47 around fibrotic lesions (1).
The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).
Specifications
Detects 47kDa.
Product Name Alternative
SerpinH1 Antibody, Colligin Antibody, Gp46 Antibody, serine proteinase inhibitor Antibody, cysteine proteinase inhibitor Antibody, collagen binding protein Antibody
Synonyms
HSP47 Antibody, Clone 1C4-1A6
UNSPSC
12352203
Gene ID
871
Swiss Prot
P50454.2
Accession Number
NP_001193943
Cellular Locus
Endoplasmic Reticulum | Endoplasmic Reticulum Lumen
Host
Mouse
Species Reactivity
Human
Immunogen
Human HSP47, full length
Target
HSP47
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
1C4-1A6
Conjugation
Unconjugated
Validated Applications
WB,ICC/IF
Purification
Protein G Purified
Limit Of Detection
1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Concentration
1 mg/ml
Dilution
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Weight
0.012
Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Precautions
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
References & Citations
1. Razzaque MS., Taquchi T., (1999) Histol Histopathol. 14 (4): 1999-212.
2. Taguchi T., et al. (2011) Acta Histochem Cytochem. 44(2):35-41.
3. Nong Z., et al. (2011) Am J Pathol. 197(5): 2189-96.
Product MSDS
https://cdn.gentaur.com/products/400/4288135/msds/smc-203s.pdf
CAS Number
9007-83-4
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