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CENP-A Antibody: Biotin

Mouse Anti-Human CENP-A Monoclonal IgG1

Product Specifications

Background

A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.

Specifications

Detects ~18kDa. Recognizes Human CENP-A.

Product Name Alternative

CENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody

Synonyms

CENP-A Antibody, Clone 5A7-2E11: Biotin

UNSPSC

12352203

Gene ID

1058

Swiss Prot

P49450

Accession Number

NP_001035891.1

Cellular Locus

Nucleus | Chromosome | Centromere | Kinetochore

Host

Mouse

Species Reactivity

Human

Immunogen

Synthetic peptide corresponding to a portion of human CENP-A

Target

CENPA

Clonality

Monoclonal

Isotype

IgG1

Clone

5A7-2E11

Conjugation

Biotin

Validated Applications

WB,IHC,ICC/IF |ELISA

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188. 3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223. 4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.

Product Datasheet

https://cdn.gentaur.com/products/400/4288098/datasheet/smc-202d-bi.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4288098/msds/smc-202d-bi.pdf

CAS Number

9007-83-4

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