Piccolo Antibody: ATTO 594

Mouse Anti-Human Piccolo Monoclonal IgG2b Kappa

Product Specifications

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Specifications

Detects ~550kDa. No cross-reactivity to Bassoon.

Product Name Alternative

ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody

Synonyms

Piccolo Antibody, Clone 6H9-B6: ATTO 594

UNSPSC

12352203

Gene ID

26875

Swiss Prot

Q9QYX7

Accession Number

NP_001104266.1

Cellular Locus

Cell Junction | Synapse

Host

Mouse

Species Reactivity

Human,Mouse,Rat

Immunogen

Human full length recombinant protein (GenBank ID: BC001304.1)

Target

Piccolo

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

6H9-B6

Conjugation

ATTO 594

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

Product Datasheet

https://cdn.gentaur.com/products/400/4287815/datasheet/smc-188d-a594.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4287815/msds/smc-188d-a594.pdf

CAS Number

9007-83-4

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