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AHA1 Antibody: FITC

Rat Anti-Mouse AHA1 Monoclonal IgG2a Kappa

Product Specifications

Background

Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Specifications

Detects ~38kDa. Can run up to 45kDa on SDS Page.

Product Name Alternative

Aha 1 Antibody, Ahsa1 Antibody, p38 Antibody, HSPC322 Antibody, C14orf3 Antibody, Activator of HSP90 ATPase Antibody, Activator of 90 kDa heat shock protein ATPase homolog 1 Antibody

Synonyms

AHA1 Antibody, Clone 25F2.D9: FITC

UNSPSC

12352203

Gene ID

217737

Swiss Prot

Q8BK64

Accession Number

NP_666148.1

Cellular Locus

Cytoplasm | Endoplasmic Reticulum

Host

Rat

Species Reactivity

Human,Mouse,Rat

Immunogen

Recombinant Full Length Mouse Aha1 Protein

Target

AHA1

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

25F2.D9

Conjugation

FITC

Validated Applications

IHC,ICC/IF

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-172 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Concentration

1.2 mg/ml

Dilution

WB (1:1000), IHC (1:100), ICC/IF (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.

Weight

0.1

Buffer

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub. 2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796. 3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723. 4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

Product Datasheet

https://cdn.gentaur.com/products/400/4287442/datasheet/smc-172d-fitc.pdf

Product MSDS

https://cdn.gentaur.com/products/400/4287442/msds/smc-172d-fitc.pdf

CAS Number

9007-83-4

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