LAMP2 Antibody, Clone GL2A7

Rat Anti-Mouse LAMP2 Monoclonal IgG1

Product Specifications

Background

LAMP2 (Lysosome-Associated Membrane Protein 2) is a transmembrane glycoprotein essential for lysosomal function and autophagy. It facilitates the maturation of autophagic vacuoles and the degradation of cellular debris. LAMP2 is expressed in lysosomes, late endosomes, and at the plasma membrane, where it may mediate cell-cell and cell-matrix interactions. In neurodegenerative diseases, LAMP2 deficiency or dysfunction impairs autophagic clearance, leading to the accumulation of toxic proteins and organelles. This contributes to neuronal death in disorders such as Danon disease, Parkinson’s disease, and Alzheimer’s disease. LAMP2 also participates in immune responses, promoting leukocyte adhesion and mast cell activation. Its role in lysosomal trafficking and neuroinflammation underscores its importance as a biomarker and therapeutic target in neurodegeneration.

CAS Number

9007-83-4

Specifications

Detects ~100-110kDa.

Product Name Alternative

LAMP2, LAMP-2, Lamp2C, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2, CD107b, CD107 antigen-like family member B, Lysosomal membrane glycoprotein type B, LGP-B, Igp110, Igp2, LampB, MAC3

Synonyms

LAMP2 Antibody, Clone GL2A7

UNSPSC

12352203

UN Code

Non-hazardous

Hazard Statement

Non-hazardous

Gene ID

16784

Swiss Prot

P17047

Accession Number

NP_001017959.1

Cellular Locus

Cell Membrane | Endosome | Lysosome | Endosome membrane | Lysosome membrane

Host

Rat

Species Reactivity

Human,Mouse,Rabbit

Immunogen

Purified preparation of mouse liver lysosomal membranes

Target

LAMP2

Clonality

Monoclonal

Isotype

IgG1

Clone

GL2A7

Conjugation

Unconjugated

Type

Monoclonal

Applications

WB | ICC/IF | IP

Validated Applications

WB,ICC/IF,IP

Field of Research

Purification

Protein G Purified

Limit Of Detection

1 µg/ml of SMC-141 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:500) ; optimal dilutions for assays should be determined by the user.

Weight

0.012

Buffer

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Precautions

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

References & Citations

1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17(5):1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132(4): 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65(1): 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61(1): 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906.

Shipping Conditions

Blue Ice or 4ºC

Storage Conditions

-20ºC

Specificity

Detects ~100-110kDa.

Background Reference 01

1. Granger B.L., et al. (1990) J. Biol. Chem. 265: 12036-12043. 2. Furuta K., et al. (1999) EMBO J. 17 (5) :1304-14. 3. Rohrer J., et al. (1996) J Cell Biol. 132 (4) : 565-76. 4. Lichter-Konecki U., et al (1999) Differentiation 65 (1) : 43-58. 5. Grutzkau A., et al. (2004) Cytometry A. 61 (1) : 62-68. 6. Kannan K., et al. (1996) Cell Immunol. 171: 10-19. 7. Tanaka Y., et al. (2000) Nature 406: 902-906.