Human Protocadherin beta-16 (PCDHB16) ELISA Kit

PCDHb16 is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins.

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Human Protocadherin beta-16 (PCDHB16) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCDHB16. No significant cross-reactivity or interference between Human PCDHB16 and analogues was observed.

This Human Protocadherin beta-16 (PCDHB16) ELISA Kit employs a two-site sandwich ELISA to quantitate PCDHB16 in samples. An antibody specific for PCDHB16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCDHB16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCDHB16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCDHB16 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.