RN185 Polyclonal Antibody

Rabbit Anti-RN185 Polyclonal Antibody

Product Specifications

Background

RNF185 (Ring Finger Protein 185) is a Protein Coding gene. Diseases associated with RNF185 include Cystic Fibrosis. Among its related pathways are Protein processing in endoplasmic reticulum and HIV Life Cycle. GO annotations related to this gene include ligase activity. An important paralog of this gene is INPP5J. E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.

CAS Number

9007-83-4

Product Name Alternative

E3 ubiquitin-protein ligase RNF185; RING finger protein 185; RING-type E3 ubiquitin transferase RNF185

Gene ID

91445

Accession Number

Q96GF1

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from part region of human RN185 protein at amino acid sequence of 121-170

Clonality

Polyclonal

Tag

RN185

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

21kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of RN185 protein

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000