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RM28 Polyclonal Antibody

Rabbit Anti-RM28 Polyclonal Antibody

Product Specifications

Background

MRPL28 (Mitochondrial Ribosomal Protein L28) is a Protein Coding gene. Among its related pathways are Mitochondrial translation and Organelle biogenesis and maintenance. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL28 encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

CAS Number

9007-83-4

Product Name Alternative

39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes; Mitochondrial large ribosomal subunit protein bL28m

Gene ID

10573

Accession Number

Q13084

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human RM28 protein at amino acid sequence of 1-80

Clonality

Polyclonal

Tag

RM28

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of RM28 protein

Observed Molecular Weight

28

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

Other Product Names

39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes; Mitochondrial large ribosomal subunit protein bL28m

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/Q13084

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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