PIF1 Polyclonal Antibody

Rabbit Anti-PIF1 Polyclonal Antibody

Product Specifications

Background

PIF1 encodes a DNA-dependent adenosine triphosphate (ATP) -metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus.

CAS Number

9007-83-4

Product Name Alternative

ATP-dependent DNA helicase PIF1; DNA repair and recombination helicase PIF1; PIF1/RRM3 DNA helicase-like protein

Gene ID

80119

Accession Number

Q9H611

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from part region of human PIF1 protein at amino acid sequence of 150-230

Clonality

Polyclonal

Tag

PIF1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

70kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of PIF1 protein

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000