NEK3 Polyclonal Antibody

Rabbit Anti-NEK3 Polyclonal Antibody

Product Specifications

Background

NEK3 encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. NIMA Related Kinase 3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

CAS Number

9007-83-4

Product Name Alternative

Serine/threonine-protein kinase Nek3; HSPK 36; Never in mitosis A-related kinase 3; NimA-related protein kinase 3

Gene ID

4752

Accession Number

P51956

Reactivity

Mouse

Immunogen

Synthesized peptide derived from part region of human NEK3 protein

Clonality

Polyclonal

Tag

NEK3

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

55kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of NEK3 protein

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000