ATP4B Polyclonal Antibody

Rabbit Anti-ATP4B Polyclonal Antibody

Product Specifications

Background

The protein encoded by ATP4B (ATPase H+/K+ Transporting Beta Subunit) belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion. ATP4B encodes the beta subunit of the gastric H+, K+-ATPase.

CAS Number

9007-83-4

Product Name Alternative

Potassium-transporting ATPase subunit beta; Gastric H (+) /K (+) ATPase subunit beta; Proton pump beta chain

Gene ID

496

Accession Number

P51164

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from part region of human ATP4B protein at amino acid sequence of 160-240

Clonality

Polyclonal

Tag

ATP4B

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

32kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of ATP4B protein

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000