ATP4A Polyclonal Antibody

Rabbit Anti-ATP4A Polyclonal Antibody

Product Specifications

Background

The protein encoded by ATP4A (ATPase H+/K+ Transporting Alpha Subunit) belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H (+) and K (+) ions across the plasma membrane. It is also responsible for gastric acid secretion. ATP4A encodes a catalytic alpha subunit of the gastric H+, K+-ATPase.

CAS Number

9007-83-4

Product Name Alternative

Potassium-transporting ATPase alpha chain 1; Gastric H (+) /K (+) ATPase subunit alpha; Proton pump

Gene ID

495

Accession Number

P20648

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from part region of human ATP4A protein at amino acid sequence of 800-880

Clonality

Polyclonal

Tag

ATP4A

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

113kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of ATP4A protein

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000